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Hormonálně řízená obrana proti hmyzím patogenům
BODLÁKOVÁ, Karolina
The aim of this bachelor thesis was to characterize the effect of one insect pathogen (entomopathogenic nematode Steinernema carpocapsae) and one venom (from the honey bee Apis mellifera) on digestive enzymes (amylases, proteases, lipases), and on adipokinetic hormone production in the brain of the cockroach Periplaneta americana . The experiments were done with the adult male and female cockroaches from a colony kept in the Insectary in the Institute of Entomology BC CAS. The pathogen/venom were applied into the cockroach body by injection 24 hours before the processing. After that the activity of digestive enzymes in the midgut, and AKH level in the brain were monitored. The results showed that the nematode has no effect on enzymes, and on AKH level as well. On the other hand, the bee venom partially increased the activity of digestive enzymes, but the role of AKH in this process in not completely clear.
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The effect of individual components of bee venom on the human body
Spurný, Jakub ; Horníková, Daniela (advisor) ; Čapková Frydrychová, Radmila (referee)
The honeybee (Apis mellifera) uses the sting apparatus to defend itself. It consists of a sting's part and a part related to the production of poison. Bee venom is secreted by special venom glands of bees. The chemical composition of the poison is a mixture of amino acides, peptides and enzymes with the various (patho)physiological effects. Some components of bee venom, for example, cause the breakdown of red blood cells, inhibit tissue excitement, increase heart rate and mean arterial pressure or cause an allergic reaction. On the other hand, the same components of bee venom also show significant positive effects on the human body. These effects have been reported in some diseases of the nervous system (Parkinson's and Alzheimer's disease), the cardiovascular system (atherosclerosis), integument (Acne vulgaris) and in the treatment of oncological diseases. For this reason, the study of bee venom components and their potential use in the therapy of various pathological conditions and diseases is important. Key words: sting apparatus; bee venom; organ system; therapy
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Studie antimikrobiálního účinku včelího jedu na druhy borelie z komplexu \kur{B. burgdorferi} sensu lato: izolace genu kódujícího antimikrobiální peptid melitin z jedové žlázy včely medonosné \kur{(Apis mellifera)}
DAVIDOVÁ, Marie
The incidence of tick borne diseases has drastically increased over the past few years. Control of tick borne pathogens, especially spirochetes from \kur{B. bugdorferi} sensu lato complex, is complicated due to a lack of vaccines against them. The emergence of bacterial strains to well established and widely used antibiotics has become a serious public health concern. Analysis of new classes of antimicrobial peptides became the subject of new investigation due to their efficacy against disease causing pathogens. Among possible candidates, melittin from honey bee venom is of particular interest. The aim of this work was to study the effect of whole bee venom on Lyme borreliosis spirochetes. The minimum concentration of the venom that possesed the inhibitory effect on borrelia growth was identified. The gene enconding melittin was isolated from bee venom gland and recombinant melittin was produced in bacterial expression system. The effect of recombinant melittin on live borrelia culture was studied.
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Medical properties of bee products
BLÁHOVÁ, Jana
Bachelor thesis is focused on the therapeutic properties of bee products. It forms a comprehensive overview of the use of bee products in the pharmacy. It describes the chemical composition and formation of bee products and improved disease and cured using Apitherapy. The thesis mentions possible contraindications of bee products.
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Representation of bee products in the nutrition of adults in the Czech Republic
ZÁHORKOVÁ, Jana
Currently at the forefront of people's healthy eating and healthy lifestyle. Bee products are a source of healthy nutrition. In the framework of representation of bee products in the nutrition of adults in the Czech Republic was through a questionnaire addressed a group of 1,086 persons age structure over 18 years old, living permanently in the Czech Republic. It was found that the best known bee products in the adult population include honey (99 %), the second most famous product is the beeswax (93 %), and knowledge of other products gradually decreases in the number of propolis (81 %), royal jelly (77 %) pollen (59 %), bee venom (45 %), bee brood (22 %). The most common honey bee products. Its consumption is 0.6 to 1 kg / person / year, when its consumption rises in interest groups, such as the beekeepers to 1.5 - 5 kg / person / year and more. The most common use of honey is its direct consumption, which is the preferred liquid consistency and type of honey - blossom (nectar) honey.
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